How to use trinocular Microscope

A trinocular microscope is a commonly used microscope with three viewing channels that allow multiple people to observe samples simultaneously.

Basic steps:

Sample Preparation: Prepare the sample to be observed and place it on the microscope viewing table. As needed, slicing, staining, or other treatments may be required to optimize the viewing of the sample.

Adjust the light source: Turn on the light source of the microscope and adjust the intensity of the light source through the brightness adjuster. Make sure the sample is properly illuminated and a clear image is obtained.

Adjust eyepiece: Adjust the focus of the eyepiece by rotating it so that it is clearly aligned with the sample surface. The eyepiece is usually located on top of the microscope.

Adjustment objective: Choose the appropriate objective as needed. Typically, trinocular microscopes are equipped with multiple objectives with different magnifications. Select the appropriate objective and align it with the sample by rotating the objective dial. To start, choose an objective with a lower magnification so that the sample can be viewed as a whole.

Adjust the focusing handwheel: Adjust the focal length of the objective by rotating the focusing handwheel to make the sample image clear. Slowly turn the handwheel and observe the changes in the image until a clear image is obtained.

Observe the sample: Use both eyes to observe the sample at the same time. Aim your eyes at the barrel of the trinocular microscope so that both eyes can see the sample image. The spacing of the barrel can be adjusted to suit the eye distance of the observer.

Adjust focus: If you need to observe different areas of the sample in detail, you can use the focusing handwheel to fine-tune it. Gradually turn the focusing handwheel as needed to keep the area to be observed clear.

Recording and Analysis: Images observed under the microscope can be recorded using a camera or other recording equipment as needed. At the same time, necessary image analysis and measurements are performed.

NBCHAO reminds you that there may be subtle differences between different models and brands of trinocular microscopes, so before use, it is recommended to refer to the relevant user manual or operation guide to ensure correct operation and obtain better observation results.