Phosphoric acid salt reagent simple operation process

Author: 同奥科技

Abstract: 本文介绍了水样中磷酸盐浓度测试的简易操作流程。根据预判的磷酸盐浓度范围，选择相应的操作步骤：0-2 mg/L使用低量程步骤，2-20 mg/L使用高量程步骤，超过20 mg/L需稀释后测定，结果乘以稀释倍数。在操作过程中，水样会变蓝色，颜色深浅反映磷酸盐浓度。此外，操作时需避免交叉污染，使用适当的移液工具，并定期校准移液枪，确保测试准确性。

Concentration prediction:Before testing the water sample, first predict the phosphate value of the water sample, and select the operation steps corresponding to the range, for example, predict the phosphate concentration value0-2mg/L, you need to select the operation procedure of 0-2mg/L range; If the phosphate concentration is predicted to be 2-20 mg/L, the 2-20 mg/L range is chosenSteps.

1. When the predicted value is in the range of 0-2mg/L

If phosphate is present, the water sample will appear blue, and the darker the color, the higher the concentration value of the water sample. Analytical procedure: phosphate L; Watch out for overrange tips!

Phosphate reagent simple operation process with Figure 1

2. When the predicted value is in the range of 2 - 20mg/L

If phosphate is present, the water sample will appear blue, and the darker the color, the higher the concentration value of the water sample. Analytical procedure: Phosphate H; Watch out for overrange tips!

The simple operation process of phosphate reagent is shown in Figure 2

3. When the predicted value exceeds 20 mgz/L

The water sample needs to be diluted to the corresponding concentration value in sections 1-2 above, and then operated according to the corresponding range, and the determination result is multiplied by the dilution factor.

4. Test steps on the computer

The simple operation process of phosphate reagent is shown in Figure 3

5. Precautions

◆ If there is phosphate, the water sample shows blue, and the darker the color, the higher the concentration value of the water sample.

◆ Pay attention to cross-contamination, use different measuring utensils for different liquids (pay attention to changing the tip when using a pipette).

◆ Use appropriate pipetting tools to take liquids, and large range measuring tools cannot measure small volumes of water samples, such as: 2mL pipettes cannot be used to remove 2mL and below liquidsFor liquids less than or equal to 2mL, it is recommended to pipette twice with a 1mL pipette.

◆ When using a pipette, it is necessary to calibrate the pipette frequently (it is recommended to do so once a week), and pay attention to replacing the tip during use.

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