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Simple operation process of sulfide reagent

Author: 同奥科技
Abstract: 本文介绍了测试水样中硫化物浓度的简易操作流程。首先,通过预判水样中的硫化物浓度,选择适当的操作步骤:0-0.8 mg/L范围使用低量程步骤,0.8-4 mg/L范围使用高量程步骤,若超出4 mg/L则需稀释后测试。测试过程中,水样颜色变化(由蓝色到深蓝色)指示浓度水平。操作时要避免交叉污染,选用适当的移液工具,并定期校准,以确保测试结果的准确性。

Concentration prediction: Before testing the water sample, the sulfide value of the water sample is first predicted, and the operation steps corresponding to the range are selected, for example, the sulfide concentration value is predicted0-0.8 mg/L, you need to select the procedure for the 0-0.8 mg/L range; If the sulfide concentration is predicted to be 0.8-4 mg/L, the 0.8-4 mg/L range is chosenProcedure.

1. When the predicted value is in the range of 0-0.8mg/L

If sulfides are present, the water sample will appear blue, and the darker the water sample, the higher the concentration value. Analytical procedure: sulfide L; Watch out for overrange tips!

The simple operation process of sulfide reagent is shown in Figure 1

2. When the predicted value is in the range of 0.8-4mg/L

If sulfides are present, the water sample will appear blue, and the darker the water sample, the higher the concentration value. Analytical Procedure: Sulfide H; Watch out for overrange tips!

The simple operation process of sulfide reagent is shown in Figure 2

3. When the predicted value exceeds 4 mg/L

The water sample needs to be diluted to the corresponding concentration value in sections 1-2 above, and then operated according to the corresponding range, and the determination result is multiplied by the dilution factor.

4. Test steps on the computer

The simple operation process of sulfide reagent is shown in Figure 3

5. Precautions

◆ If there is sulfide, the water sample shows blue, and the darker the color, the higher the concentration value of the water sample.

◆ Pay attention to cross-contamination, use different measuring utensils for different liquids (pay attention to changing the tip when using a pipette).

◆ Use appropriate pipetting tools to take liquids, and large range measuring tools cannot measure small volumes of water samples, such as: 2mL pipettes cannot be used to remove 2mL and below liquidsFor liquids less than or equal to 2mL, it is recommended to pipette twice with a 1mL pipette.

◆ When using a pipette, it is necessary to calibrate the pipette frequently (it is recommended to do so once a week), and pay attention to replacing the tip during use.


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