Application of six-hole Water Bath in rapid dehydration of endoscopic biopsy tissue

Our department uses a six-hole Water Bath to dehydrate biopsy tissues such as endoscopes, which greatly saves dehydration time, improves the speed of film production , and sends them for inspection at a high speed, thus shortening the time for issuing pathological diagnosis reports and satisfying the needs of patients. The needs of patients and their families were welcomed . This method is simple, easy to master, suitable for medical institutions at all levels, and the cost of is low, so it has certain practical value and economic value.

1 Materials and methods

1.1 Materials (1) Endoscopic biopsy tissue: including various organs and tissues taken by gastroscope, colonoscope, cystoscope, fiberoptic bronchoscope, etc. (2) Needle aspiration biopsy tissue: including liver, kidney, lung, prostate, lymph node, etc. and tumor puncture tissue .

1.2 Method

1 h before dehydration of the samples, turn on the power of the six-hole Water Bath, and heat the Water Bath to the set temperature of 68°C to 70°C. After taking the specimens, dehydrate the tissues sequentially . After the tissue dehydration is completed, soak in wax for 1-2 hours, and then the sections can be embedded. Note: There is 600ml of stearic acid paraffin solution in the wax bath (the ratio of stearic acid to paraffin volume or weight is 2:3, that is, every 100 ml of stearic acid paraffin solution contains 40 ml of stearic acid and 60 ml of paraffin wax. ), the temperature of the wax dipping solution must be kept at 68°C to 70°C, and the wax dipping liquid can be left in a metal container and placed in an Incubator, or the wax dipping liquid can be left directly in the storage box of the embedding machine (if the volume is too large , the amount of wax dipping solution can be appropriately increased.)

2 results

The dehydration process took 40 minutes in total, soaked in wax for about 1.5 hours, and collected samples once a day from Monday to Friday. Considering the gradual reduction of ethanol concentration, we replaced the reagents once every two weeks , including the replacement of stearic acid paraffin solution. However, sometimes in actual work, according to the dehydration and sectioning of the tissue, we replace the AAF solution in the first channel after two weeks of dehydration, remove the 95% ethanol in the second channel , and replace the 95% ethanol in the third channel. Move 95% ethanol forward to the second channel, and so on. After the original sixth channel is moved forward to the fifth channel, replace the sixth channel with a new solution. When the amount of reagent solution decreases, it can be increased to a sufficient amount accordingly. ; Stearic acid paraffin solution, because the tissue and its wrapping paper are continuously taken away and lost after being soaked in wax, it can be added according to the ratio of the two to reduce costs and waste. Our department has been using this method for more than 4 years, and the film production effect has been very good. No adverse phenomena such as tissue hardness and brittleness and cell shrinkage under the microscope have been found, and the harm to the human body caused by the xylene used in the past for dehydration and transparency has been avoided.

3 Discussion

Our department collects the gastroscopy and other endoscopes and needle biopsy tissues sent in at around 11:00 every morning , and the collection is completed at about 11:15, and then the specimens are put into the heated "six-hole Water Bath" "The cylinders are dehydrated sequentially, and the dehydrated specimens are soaked in wax before leaving work at noon every day , and are embedded, made into slices and sent for pathological examination when they go to work in the afternoon. The patient can get the pathological examination report in the next morning, especially for some patients. For special patients, the diagnosis result can be known in the afternoon of the same day, which greatly facilitates the patients and enables them to receive timely and effective treatment.