Why is a Spectrophotometer calibrated? What is the principle?

Spectrophotometer calibration is the process by which a scientific instrument called a Spectrophotometer is calibrated to confirm that it is working properly. This is important because it ensures that the measurements obtained with the instrument are accurate. The procedure varies slightly from instrument to instrument, and most manufacturers provide detailed calibration guidelines in the user manual so that people know how to properly calibrate the device. When this procedure is performed, the person performing the procedure will need to make a note in the logbook that came with the device and in their experimental records so that people know when and by whom the device was last calibrated and processed.

Avoid performing Spectrophotometer calibration during the warm-up phase, as this will lead to loss of settings.

A Spectrophotometer can both emit and receive light. This device is used to analyze a sample of test material by passing light through the sample and reading the intensity of the wavelength. Different samples affect light in different ways, and a researcher or technician can learn more about the materials in a test sample by observing how light behaves as it passes through the sample. Calibration of the Spectrophotometer is necessary to confirm that the results are accurate.

In Spectrophotometer calibration, a reference solution is used to zero the device. This solution provides a base or zero reading. The device is calibrated by placing a reference solution in the Spectrophotometer , zeroing the set point, and running the instrument. Spectrophotometry can then be performed on a sample of the actual test material to confirm that the machine is calibrated and working properly.

In a single-beam Spectrophotometer , a single beam of light is produced, requiring recalibration of the unit each time it is used. In a dual-beam Spectrophotometer , beams of light can pass through a test sample and a reference sample simultaneously, producing two sets of results that can be used for reference and calibration. In either case, calibration of the Spectrophotometer can be done in the laboratory by the person operating the machine. If a machine develops a serious problem, it can be sent to the manufacturer for maintenance, repair, and potential replacement.

In order for the Spectrophotometer to work properly, it needs to be warmed up before use. Many devices require a warm-up time of about 10 minutes. It is important to avoid Spectrophotometer calibration during the warm-up phase, as this will result in the setup being canceled. It is also important to know that for certain types of wavelengths, special filters and accessories may be required for the device to work.