How is the content of non-fibroblasts determined?

How is the non-fibroblast content measured?

Methods for determining the content of non-fibroblasts mainly include the following:

• Microscopic counting method: After the sample is properly processed, the cells are counted under a microscope, and the ratio of the number of non-fibrotic cells to the total number of cells is calculated to obtain the content of non-fibrotic cells.

• Chemical method: Dissolve fiber components such as cellulose by a certain chemical method, and the remaining substances are non-fibrous cells, which can be weighed to calculate the content of non-fibrous cells.

• Optical microscope image processing method : After the sample is prepared into thin slices, the image is obtained under the microscope, and the non-fibrous cells and fibers are segmented and calculated using image processing software, so as to obtain the content of non-fibrous cells.

• Physical property analysis method: Analyze the sample by a physical property analyzer, such as air-water specific gravity, centrifugation, etc., and divide the sample into different components to obtain the content of non-fibroblasts.

These methods above can be used alone or in combination to obtain a more comprehensive and accurate measurement of non-fibroblast content. Different methods are suitable for different sample types and experimental requirements, and the appropriate method needs to be selected according to the specific situation.