Inject the water sample or the pretreated water sample into the culture bottle, and put carbon dioxide absorbent (NaOH) into the absorption cup at the mouth of the bottle, then seal the culture bottle and place it in a special biochemical Incubator. After cultivating at a constant temperature of 20±1°C for five days, the water sample in the bottle undergoes a biological oxidation reaction at a certain stirring speed. The organic matter is converted into oxides of nitrogen, carbon and sulfur, and carbon dioxide gas is produced to be absorbed by sodium hydroxide, while the oxygen in the upper space of the culture bottle continuously supplies the oxygen demand of the microorganisms in the water sample, and a pressure difference is generated in the culture bottle. By detecting the height of mercury in the glass tube, the BOD value of the water sample within the specified measurement period can be calculated.
