Dark field lighting is also called dark field lighting. Its characteristics are different from bright field. It does not directly observe the illumination light, but observes the light reflected or diffracted by the object being inspected. Therefore, the field of view becomes a dark background, while the inspected object appears as a bright image. A special accessory required for dark field observation is a dark field condenser. Its characteristic is that it does not allow the light beam to pass through the object being inspected from bottom to top, but changes the light to
Change the path so that it is directed obliquely towards the object being inspected, so that the illumination light does not directly enter the objective lens, and the light reflected or diffracted on the surface of the object being inspected is used to form a bright image.
The dark field observation microscope is suitable for tiny living bodies that cannot be seen under ordinary microscopes. It does not stain biological samples and observes their shape and outline. It is widely used in scientific research, teaching, medical treatment, epidemic prevention, agriculture and animal husbandry and other industries.
The bright and dark field microscopes use electric light sources with continuously adjustable brightness. The hinged lens barrel is tilted at 30° and can rotate freely 360 degrees. ;
The hinged trinocular tube is tilted at 30°, with a split prism push-pull rod that can rotate 360 ââdegrees;
The CCD channel of the trinocular tube is equipped with a multi-head threaded image fine-tuning mechanism. The fine-tuning range is 10mm (including CCD interface).
eyepiece:
| category | gain | Direct field of view |
| eyepiece | 10X | Ï18mm |
| 16X | Ï11mm |
Objective lens:
| category | gain | numerical aperture | working distance |
| objective lens | 4X | 0.1NA | 37.5mm |
| 10X | 0.25NA | 7.31mm | |
| 40X | 0.65NA | 0.63mm | |
| 100X (oil) | 1.25NA | 0.18mm |