The GB/T 23737-2009 standard specifies the use of Ion exchange chromatography, IEC for the determination of free canavanine in feeds. The standard applies to the analysis of single feeds and compound feeds, and the quantification limit is 15 mg/kg. The method principle involves extracting free canavanine from the sample by sulfosalicylic acid, filtrating and bandwidth evaluation, separating it using ion exchange Chromatography Column, and then derivatization by indenhydrin column, determined at a wavelength of 570 nm. The standard requires the use of analytically pure reagents and water in accordance with GB/T 6682, and details the preparation methods of the required reagents, including muriatic acid, sulfosalicylic acid, sodium citrate buffer, and indanhydrin solution.
| Status | Active |
|---|---|
| CCS | B46 | ICS | 65.120 |
| Release Date | 2009-05-12 00:00:00 | Implementation Date | 2009-09-01 00:00:00 |